Synthesis of Gentamicin Minor Components: Gentamicin B1 and Gentamicin X2

Gentamicin B1 is a minor gentamicin component with major nonsense mutation suppression activity.

Nonsense mutations underlie about 10% of rare genetic disease cases. They introduce a premature termination codon (PTC) and prevent the formation of full-length protein. Pharmaceutical gentamicin, a mixture of several related aminoglycosides, is a frequently used antibiotic in humans that can induce PTC readthrough and suppress nonsense mutations at high concentrations. However, testing of gent...

Delineating the Biosynthesis of Gentamicin X2, the Common Precursor of the Gentamicin C Antibiotic Complex

Gentamicin C complex is a mixture of aminoglycoside antibiotics used worldwide to treat severe Gram-negative bacterial infections. Despite its clinical importance, the enzymology of its biosynthetic pathway has remained obscure. We report here insights into the four enzyme-catalyzed steps that lead from the first-formed pseudotrisaccharide gentamicin A2 to gentamicin X2, the last common interme...

Gentamicin injection

Silica-Gentamicin Nanohybrids: Synthesis and Antimicrobial Action

Orthopedic applications commonly require the administration of systemic antibiotics. Gentamicin is one of the most commonly used aminoglycosides in the treatment and prophylaxis of infections associated with orthopedic applications, but gentamicin has a short half-life. However, silica nanoparticles (SiO₂ NPs) can be used as elegant carriers for antibiotics to prolong their release. Our goal is...

Gentamicin elution profile of SmartSet GHV Gentamicin Bone Cement

Introduction There is no doubt that, since the invention of artificial joint replacement, many thousands of people have enjoyed freedom from disabling pain and immobility. However, this remarkable success story is marred by the incidence of local sepsis following surgery, which can lead to prosthesis loosening and the need for revision surgery. In fact, deep wound infections are the most seriou...